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Addgene

pcDNA3-HA-ROC2
(Plasmid #19899)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5437
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RING finger protein 2 (ROC2)
  • Alt name
    ROC2, SAG, CKBBP1; RNF7
  • Alt name
    RING finger protein 7
  • Alt name
    Regulator of Cullins 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    339
  • GenBank ID
    AF142060
  • Entrez Gene
    RNF7 (a.k.a. CKBBP1, ROC2, SAG, rbx2)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-ROC2 was a gift from Yue Xiong (Addgene plasmid # 19899 ; http://n2t.net/addgene:19899 ; RRID:Addgene_19899)

  • For your References section:

    ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Mol Cell. 1999 Apr . 3(4):535-41. 10.1016/S1097-2765(00)80482-7 PubMed 10230407
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