pcDNA3-HA2-ROC1
(Plasmid #19897)

• Depositing Lab: Yue Xiong
• Publication: Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Backbone

• Vector backbone: pcDNA3-HA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RING finger protein 1 (ROC1)
• Alt name: ROC1, RNF75, RBX1
• Alt name: RBX1 ring-box 1
• Alt name: Regulator of Cullins 1
• Species: H. sapiens (human)
• Insert Size (bp): 323
• GenBank ID: AF142059
• Entrez Gene: RBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry