pcDNA3-myc3-CUL1
(Plasmid #19896)

• Depositing Lab: Yue Xiong
• Publication: Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5437
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cullin 1
• Alt name: CUL1
• Species: H. sapiens (human)
• Insert Size (bp): 2260
• GenBank ID: NP_003583
• Entrez Gene: CUL1
• Tag / Fusion Protein:
  • myc3 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

When we made this plasmid, we put a Nco1 site at the start codon (original cDNA was CAATGTCG but they mutated to CcATGgCG). It made an S2A mutation. There is also a K689E mutation. These mutations have no effect on protein function.