pcDNA3-myc-CUL5
(Plasmid #19895)

• Depositing Lab: Yue Xiong
• Publication: Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Backbone

• Vector backbone: pcDNA3-myc
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5437
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cullin 5
• Alt name: CUL5
• Species: H. sapiens (human)
• Insert Size (bp): 2313
• GenBank ID: NM_003478
• Entrez Gene: CUL5 (a.k.a. CUL-5, VACM-1, VACM1)
• Tag / Fusion Protein:
  • myc3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry