pcDNA3-myc-CUL3
(Plasmid #19893)

• Depositing Lab: Yue Xiong
• Publication: Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Backbone

• Vector backbone: pcDNA3-myc
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5512
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cullin 3
• Alt name: CUL3
• Species: H. sapiens (human)
• Insert Size (bp): 2304
• GenBank ID: NM_003590
• Entrez Gene: CUL3 (a.k.a. CUL-3, NEDAUS, PHA2E)
• Tag / Fusion Protein:
  • 3xmyc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Y201C mutation detected in Addgene sequence. Xiong lab says that the plasmid used in the publication also had Y201C mutation and it is not known to affect function.