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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 19892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3-myc3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5437
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Vector typeMammalian Expression
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Selectable markersG418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecullin 2
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Alt nameCUL2
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SpeciesH. sapiens (human)
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Insert Size (bp)2235
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GenBank IDNM_003591
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Entrez GeneCUL2
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Tag
/ Fusion Protein
- myc3 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-myc3-CUL2 was a gift from Yue Xiong (Addgene plasmid # 19892 ; http://n2t.net/addgene:19892 ; RRID:Addgene_19892) -
For your References section:
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Mol Cell. 1999 Apr . 3(4):535-41. 10.1016/S1097-2765(00)80482-7 PubMed 10230407
Map uploaded by the depositor.
