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GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3
(Plasmid #198914)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198914 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen Life Technologies
  • Backbone size w/o insert (bp) 5352
  • Total vector size (bp) 13239
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    LB medium
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cav2.2
  • Alt name
    calcium channel alpha1B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7887
  • Mutation
    exon 18a deleted, exon 19 starts 3 nucleotides earlier giving an extra Glutamine
  • Entrez Gene
    CACNA1B (a.k.a. BIII, CACNL1A5, CACNN, Cav2.2, DYT23, NEDNEH)
  • Promoter CMV
  • Tag / Fusion Protein
    • N-terminal GFP, internal double HA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CTGGCTAACTAGAGAACC
  • 3′ sequencing primer ATCAGCGAGCTCAGCAT
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene item ID 62574, huCaV2.2 (pSAD442-1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3 was a gift from Annette Dolphin (Addgene plasmid # 198914 ; http://n2t.net/addgene:198914 ; RRID:Addgene_198914)

  • For your References section:

    The Interplay Between Splicing of Two Exon Combinations Differentially Affects Membrane Targeting and Function of Human CaV2.2. Dahimene S, Page KM, Nieto-Rostro M, Pratt WS, Dolphin AC. Function (Oxf). 2023 Oct 19;5(1):zqad060. doi: 10.1093/function/zqad060. eCollection 2024. 10.1093/function/zqad060 PubMed 38020068
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