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Addgene

Opto-ACTU−
(Plasmid #198869)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherryN1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 6283
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rad17 polyanionic tail (-14)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    101
  • Promoter CMV
  • Tag / Fusion Protein
    • CRY2PHR-mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer mCherry-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Opto-ACTU− was a gift from Peter Devreotes (Addgene plasmid # 198869 ; http://n2t.net/addgene:198869 ; RRID:Addgene_198869)

  • For your References section:

    Spatiotemporal dynamics of membrane surface charge regulates cell polarity and migration. Banerjee T, Biswas D, Pal DS, Miao Y, Iglesias PA, Devreotes PN. Nat Cell Biol. 2022 Oct;24(10):1499-1515. doi: 10.1038/s41556-022-00997-7. Epub 2022 Oct 6. 10.1038/s41556-022-00997-7 PubMed 36202973
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