pFRT/TO/FLAG/HA-DEST TNRC6A
(Plasmid #19883)

• Depositing Lab: Thomas Tuschl
• Publication: Landthaler et al RNA. 2008 Oct 31. ():.

Backbone

• Vector backbone: pFRT/TO/FLAG/HA-DEST
• Backbone size w/o insert (bp): 5421
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trinucleotide repeat containing 6A
• Alt name: TNRC6A; GW1; GW182; TNRC6; CAGH26; FLJ22043; KIAA1460; MGC75384; DKFZp666E117
• Species: H. sapiens (human)
• Insert Size (bp): 5130
• GenBank ID: NM_014494
• Entrez Gene: TNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
• Tag / Fusion Protein:
  • FLAG/HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid does not contain the 2x tet operator sequence normally in the pFRT-TO backbone.