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Addgene

pFRT/TO/FLAG/HA-DEST TNRC6A
(Plasmid #19883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRT/TO/FLAG/HA-DEST
  • Backbone size w/o insert (bp) 5421
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trinucleotide repeat containing 6A
  • Alt name
    TNRC6A; GW1; GW182; TNRC6; CAGH26; FLJ22043; KIAA1460; MGC75384; DKFZp666E117
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5130
  • GenBank ID
    NM_014494
  • Entrez Gene
    TNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid does not contain the 2x tet operator sequence normally in the pFRT-TO backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/TO/FLAG/HA-DEST TNRC6A was a gift from Thomas Tuschl (Addgene plasmid # 19883 ; http://n2t.net/addgene:19883 ; RRID:Addgene_19883)

  • For your References section:

    Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T. RNA. 2008 Oct 31. ():. 10.1261/rna.1351608 PubMed 18978028
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