pJR014
(Plasmid
#198813)
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PurposeNatural light-gated anion channels. In vivo inhibition of neurons using blue light.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPD117.01
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Backbone manufacturerFire Lab C. elegans Vector Kit
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name15xUAS::GtACR2::SL2::GFP::let-858 3'UTR
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SpeciesC. elegans (nematode)
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Insert Size (bp)2481
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJR014 was a gift from Paul Sternberg (Addgene plasmid # 198813 ; http://n2t.net/addgene:198813 ; RRID:Addgene_198813) -
For your References section:
A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans sensory neurons. Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW. Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 10.1073/pnas.2221680120. Epub 2023 Dec 14. 10.1073/pnas.2221680120 PubMed 38096407