pLV[TetOn]-Puro-TRE3G>mAscl1
(Plasmid
#198755)
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PurposeVector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 198755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV[TetOn]-Puro-TRE3G
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Backbone manufacturerVectorbuilder
- Backbone size w/o insert (bp) 7692
- Total vector size (bp) 8388
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAscl1
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SpeciesM. musculus (mouse)
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Insert Size (bp)695
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GenBank IDNM_008553.5
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Entrez GeneAscl1 (a.k.a. ASH1, Mash1, bHLHa46)
- Promoter TRE3G
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV[TetOn]-Puro-TRE3G>mAscl1 was a gift from Nael Nadif Kasri (Addgene plasmid # 198755 ; http://n2t.net/addgene:198755 ; RRID:Addgene_198755) -
For your References section:
Cadherin-13 is a critical regulator of GABAergic modulation in human stem-cell-derived neuronal networks. Mossink B, van Rhijn JR, Wang S, Linda K, Vitale MR, Zoller JEM, van Hugte EJH, Bak J, Verboven AHA, Selten M, Negwer M, Latour BL, van der Werf I, Keller JM, Klein Gunnewiek TM, Schoenmaker C, Oudakker A, Anania A, Jansen S, Lesch KP, Frega M, van Bokhoven H, Schubert D, Nadif Kasri N. Mol Psychiatry. 2022 Jan;27(1):1-18. doi: 10.1038/s41380-021-01117-x. Epub 2021 May 10. 10.1038/s41380-021-01117-x PubMed 33972691
Map uploaded by the depositor.
