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v3em-Cterm-PE2max-∆RNaseH-dualU6
(Plasmid #198735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p601 modified
  • Total vector size (bp) 4967
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NpuC-CtermPE2max∆RNaseH
  • Insert Size (bp)
    2850
  • Promoter Cbh

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGCGAGTCAGTGAGCGAG
  • 3′ sequencing primer GTTTTCCCAGTCACGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    v3em-Cterm-PE2max-∆RNaseH-dualU6 was a gift from David Liu (Addgene plasmid # 198735 ; http://n2t.net/addgene:198735 ; RRID:Addgene_198735)

  • For your References section:

    Efficient prime editing in mouse brain, liver and heart with dual AAVs. Davis JR, Banskota S, Levy JM, Newby GA, Wang X, Anzalone AV, Nelson AT, Chen PJ, Hennes AD, An M, Roh H, Randolph PB, Musunuru K, Liu DR. Nat Biotechnol. 2023 May 4. doi: 10.1038/s41587-023-01758-z. 10.1038/s41587-023-01758-z PubMed 37142705
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