v3em-Nterm-PE2max
(Plasmid #198734)

• Purpose: AAV genome encoding N-terminal PE2max
• Depositing Lab: David Liu
• Publication: Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038/s41587-023-01758-z.

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Backbone

• Vector backbone: p601 modified
• Total vector size (bp): 4739
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Nterm PE2max-NpuN
• Insert Size (bp): 3500
• Promoter: Cbh

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCGAGTCAGTGAGCGAG
• 3′ sequencing primer: GTTTTCCCAGTCACGAC

Resource Information

• Supplemental Documents:
  • JRD340-v3em-Nterm-PE2max.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry