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Addgene

pCAG-RhoA*
(Plasmid #198716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 4792
  • Total vector size (bp) 5374
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RhoA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    582
  • Mutation
    It has mutations that is resistant to the RhoA gRNAs (#198718, #198719, #198720). Amino acid sequence is not affected.
  • Entrez Gene
    Rhoa (a.k.a. Arha, Arha1, Arha2)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/625616 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-RhoA* was a gift from Takeshi Imai (Addgene plasmid # 198716 ; http://n2t.net/addgene:198716 ; RRID:Addgene_198716)

  • For your References section:

    Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7. doi: 10.1016/j.devcel.2023.05.004. Epub 2023 Jun 7. 10.1016/j.devcel.2023.05.004 PubMed 37290446
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