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Addgene

Lenti-SAM-Puro
(Plasmid #198713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lenti-LucOS
  • Backbone manufacturer
    Addgene 22777
  • Backbone size w/o insert (bp) 5818
  • Total vector size (bp) 9616
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    U6-MS2-gRNAscaffold
  • Alt name
    U6 promoter
  • Alt name
    MS2 stem loops
  • Alt name
    guide RNA cloning cassette
  • Insert Size (bp)
    412
  • Promoter U6

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATCAGTGTGAGGGAGTGTAAAGCTGGTTT
  • 3′ sequencing primer AGGCCTCGGGATTCCTAGGAACAGCGGTTT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    EF1a-MPH-Puro
  • Alt name
    EF1a promoter
  • Alt name
    MS2 binding protein and p65, HSF1 transcriptional activators
  • Alt name
    Puromycin resistance gene
  • Insert Size (bp)
    2610
  • Promoter EF1a

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAACCGCTGTTCCTAGGAATCCCGAGGCCT
  • 3′ sequencing primer AGAGTAATTCAACCCCAAACAACAACGTTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-SAM-Puro was a gift from Tyler Jacks (Addgene plasmid # 198713 ; http://n2t.net/addgene:198713 ; RRID:Addgene_198713)

  • For your References section:

    Lymphocyte networks are dynamic cellular communities in the immunoregulatory landscape of lung adenocarcinoma. Gaglia G, Burger ML, Ritch CC, Rammos D, Dai Y, Crossland GE, Tavana SZ, Warchol S, Jaeger AM, Naranjo S, Coy S, Nirmal AJ, Krueger R, Lin JR, Pfister H, Sorger PK, Jacks T, Santagata S. Cancer Cell. 2023 May 8;41(5):871-886.e10. doi: 10.1016/j.ccell.2023.03.015. Epub 2023 Apr 13. 10.1016/j.ccell.2023.03.015 PubMed 37059105
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