His6_MBP_FXR1P
(Plasmid
#198694)
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PurposeExpression of human FXR1 protein in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMCSG9
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFXR1P
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SpeciesH. sapiens (human)
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Insert Size (bp)1617
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Entrez GeneFXR1 (a.k.a. CMYP9A, CMYP9B, FXR1P, MYOPMIL, MYORIBF)
- Promoter T7
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Tag
/ Fusion Protein
- MBP (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His6_MBP_FXR1P was a gift from Simpson Joseph (Addgene plasmid # 198694 ; http://n2t.net/addgene:198694 ; RRID:Addgene_198694) -
For your References section:
A simple procedure for bacterial expression and purification of the fragile X protein family. Edwards M, Xu M, Joseph S. Sci Rep. 2020 Sep 28;10(1):15858. doi: 10.1038/s41598-020-72984-7. 10.1038/s41598-020-72984-7 PubMed 32985615