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His6_MBP_FMRP_P451S
(Plasmid #198693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MCSG9
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FMR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1896
  • Mutation
    changed proline 451 to serine
  • Entrez Gene
    FMR1 (a.k.a. FMRP, FRAXA, POF, POF1)
  • Promoter T7
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6_MBP_FMRP_P451S was a gift from Simpson Joseph (Addgene plasmid # 198693 ; http://n2t.net/addgene:198693 ; RRID:Addgene_198693)

  • For your References section:

    A simple procedure for bacterial expression and purification of the fragile X protein family. Edwards M, Xu M, Joseph S. Sci Rep. 2020 Sep 28;10(1):15858. doi: 10.1038/s41598-020-72984-7. 10.1038/s41598-020-72984-7 PubMed 32985615
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