pEM.P05L
(Plasmid
#198649)
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PurposeEasy-MISE toolkit pEM-plasmid containing CYC1 promoter with EF protruding ends
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGA-blue
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Backbone manufacturerIndBioTech Lab
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Vector typeYeast Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCYC1
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning Method: Goldon Gate Assembly
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEM.P05L was a gift from Paola Branduardi (Addgene plasmid # 198649 ; http://n2t.net/addgene:198649 ; RRID:Addgene_198649) -
For your References section:
Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in Saccharomyces cerevisiae. Maestroni L, Butti P, Milanesi R, Pagliari S, Campone L, Serra I, Branduardi P. ACS Synth Biol. 2023 Apr 14. doi: 10.1021/acssynbio.3c00015. 10.1021/acssynbio.3c00015 PubMed 37058502