pBad-HaloGFP-153-154
(Plasmid
#198604)
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PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198604 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePBad
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaloGFP-153-154
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SpeciesSynthetic
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Insert Size (bp)1620
- Promoter araBAD promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBad-HaloGFP-153-154 was a gift from Robert Campbell (Addgene plasmid # 198604 ; http://n2t.net/addgene:198604 ; RRID:Addgene_198604) -
For your References section:
Chemigenetic indicators based on synthetic chelators and green fluorescent protein. Zhu W, Takeuchi S, Imai S, Terada T, Ueda T, Nasu Y, Terai T, Campbell RE. Nat Chem Biol. 2023 Jan;19(1):38-44. doi: 10.1038/s41589-022-01134-z. Epub 2022 Sep 22. 10.1038/s41589-022-01134-z PubMed 36138142