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Addgene

UG75-ADH1-DGK1-3HA
(Plasmid #198595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    UG75
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DGK1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    DGK1 (a.k.a. HSD1)
  • Tag / Fusion Protein
    • 3HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UG75-ADH1-DGK1-3HA was a gift from Zhiping Xie (Addgene plasmid # 198595 ; http://n2t.net/addgene:198595 ; RRID:Addgene_198595)

  • For your References section:

    Excess diacylglycerol at the endoplasmic reticulum disrupts endomembrane homeostasis and autophagy. Li D, Yang SG, He CW, Zhang ZT, Liang Y, Li H, Zhu J, Su X, Gong Q, Xie Z. BMC Biol. 2020 Aug 28;18(1):107. doi: 10.1186/s12915-020-00837-w. 10.1186/s12915-020-00837-w PubMed 32859196
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