pGBT9-FHIP-L
(Plasmid
#198528)
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PurposeExpression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGBT9
- Backbone size w/o insert (bp) 5524
- Total vector size (bp) 8637
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFHIP-L
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Alt nameFAM160A1
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SpeciesH. sapiens (human)
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Insert Size (bp)3123
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Entrez GeneFHIP1A (a.k.a. FAM160A1, FHIP-L)
- Promoter ADH1
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Tag
/ Fusion Protein
- GAL4-DNA binding domain fragment (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer GAL4 DNA binding domain forward
- 3′ sequencing primer GAL4 DNA binding domain reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBT9-FHIP-L was a gift from Juan Bonifacino (Addgene plasmid # 198528 ; http://n2t.net/addgene:198528 ; RRID:Addgene_198528) -
For your References section:
The FTS-Hook-FHIP (FHF) complex interacts with AP-4 to mediate perinuclear distribution of AP-4 and its cargo ATG9A. Mattera R, Williamson CD, Ren X, Bonifacino JS. Mol Biol Cell. 2020 Apr 15;31(9):963-979. doi: 10.1091/mbc.E19-11-0658. Epub 2020 Feb 19. 10.1091/mbc.E19-11-0658 PubMed 32073997