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Addgene

pGBT9-FHIP
(Plasmid #198527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT9
  • Backbone size w/o insert (bp) 5524
  • Total vector size (bp) 8439
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FHIP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2919
  • Mutation
    silent mutations in codons 353 and 651 as well as P599H- Please see depositor comments
  • Entrez Gene
    FHIP1B (a.k.a. C11orf56, FAM160A2, FHIP)
  • Promoter ADH1
  • Tag / Fusion Protein
    • GAL4-DNA binding domain fragment (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer GAL4 DNA binding domain forward
  • 3′ sequencing primer GAL4 DNA binding domain reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there is a single mutation P599H in FHIP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT9-FHIP was a gift from Juan Bonifacino (Addgene plasmid # 198527 ; http://n2t.net/addgene:198527 ; RRID:Addgene_198527)

  • For your References section:

    The FTS-Hook-FHIP (FHF) complex interacts with AP-4 to mediate perinuclear distribution of AP-4 and its cargo ATG9A. Mattera R, Williamson CD, Ren X, Bonifacino JS. Mol Biol Cell. 2020 Apr 15;31(9):963-979. doi: 10.1091/mbc.E19-11-0658. Epub 2020 Feb 19. 10.1091/mbc.E19-11-0658 PubMed 32073997
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