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Addgene

pGBT9-Hook1
(Plasmid #198523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT9
  • Backbone size w/o insert (bp) 5524
  • Total vector size (bp) 7712
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hook1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2189
  • Mutation
    silent substitution in codon 620 to eliminate internal BamH1 site
  • Entrez Gene
    HOOK1 (a.k.a. HK1)
  • Promoter ADH1
  • Tag / Fusion Protein
    • GAL4-DNA binding domain fragment (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer GAL4 DNA binding domain forward
  • 3′ sequencing primer GAL4 DNA binding domain reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT9-Hook1 was a gift from Juan Bonifacino (Addgene plasmid # 198523 ; http://n2t.net/addgene:198523 ; RRID:Addgene_198523)

  • For your References section:

    The FTS-Hook-FHIP (FHF) complex interacts with AP-4 to mediate perinuclear distribution of AP-4 and its cargo ATG9A. Mattera R, Williamson CD, Ren X, Bonifacino JS. Mol Biol Cell. 2020 Apr 15;31(9):963-979. doi: 10.1091/mbc.E19-11-0658. Epub 2020 Feb 19. 10.1091/mbc.E19-11-0658 PubMed 32073997
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