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pRevTRE MKL1 S449A/T450A/S454A
(Plasmid #19847)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19847 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRev TRE
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MKL1 S449A/T450A/S454A
  • Alt name
    MAL, BSAC, MRTF-A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2800
  • Mutation
    changed Serine 449, Threonine 450 and Serine 454 to Alanines.
  • Entrez Gene
    MRTFA (a.k.a. BSAC, MAL, MKL, MKL1, MRTF-A)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 3xFlag in the vector is from Sigma, p3xFlag-CMV-7.1 has variant flag sequences (DYKDHD) and is recognized well by their anti-flag monoclonal M2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRevTRE MKL1 S449A/T450A/S454A was a gift from Ron Prywes (Addgene plasmid # 19847 ; http://n2t.net/addgene:19847 ; RRID:Addgene_19847)

  • For your References section:

    Serum-induced phosphorylation of the SRF coactivator MKL1 by the ERK1/2 pathway inhibits its nuclear localization. Muehlich S, Wang R, Lee SM, Lewis TC, Dai C, Prywes R. Mol Cell Biol. 2008 Aug 11. ():. 10.1128/MCB.00427-08 PubMed 18694962
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