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Addgene

eZiPro W83F
(Plasmid #198439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11a
  • Backbone manufacturer
    MilliporeSigma (Novagen)
  • Backbone size w/o insert (bp) 5677
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    enzymatically cleavable linked Zika virus NS2B-NS3
  • Alt name
    enzymatically cleavable linked ZIKV NS2Bcf-NS3pro
  • Species
    Zika Virus (KJ776791.2)
  • Insert Size (bp)
    786
  • Mutation
    W83F
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His6-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 prom
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eZiPro W83F was a gift from Ute Hellmich & Tanja Schirmeister (Addgene plasmid # 198439 ; http://n2t.net/addgene:198439 ; RRID:Addgene_198439)

  • For your References section:

    The effects of allosteric and competitive inhibitors on ZIKV protease conformational dynamics explored through smFRET, nanoDSF, DSF, and (19)F NMR. Maus H, Hammerschmidt SJ, Hinze G, Barthels F, Perez Carrillo VH, Hellmich UA, Basche T, Schirmeister T. Eur J Med Chem. 2023 Oct 5;258:115573. doi: 10.1016/j.ejmech.2023.115573. Epub 2023 Jun 21. 10.1016/j.ejmech.2023.115573 PubMed 37379675
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