pGST2-GST-TEV-OPTN
(Plasmid #198428)

• Purpose: Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)
• Depositing Lab: Sascha Martens
• Publication: Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021.

Backbone

• Vector backbone: pGST2
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6694
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: optineurin
• Alt name: NRP
• Alt name: FIP2
• Species: H. sapiens (human)
• GenBank ID: NC_000010.11
• Entrez Gene: OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
• Tags / Fusion Proteins:
  • GST (N terminal on insert)
  • TEV (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: atGAAAACCTGTATTTTCA
• 3′ sequencing primer: GTTAaatgatgcaatccatcacg

Resource Information

• Supplemental Documents:
  • SMC1587-pGST2-GST-TEV-hOPTN.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry