pDP085
(Plasmid #198425)

• Purpose: pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120
• Depositing Lab: Markus Schmid
• Publication: You et al Nat Commun. 2017 May 17;8:15120. doi: 10.1038/ncomms15120.

Backbone

• Vector backbone: pGreen
• Vector type: Plant Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E. coli BirA
• Promoter: pUBQ10

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GTAAAACGACGGCCAGTG
• 3′ sequencing primer: CAGGAAACAGCTATGACC

Resource Information

• Supplemental Documents:
  • pDP085.gbk
• A portion of this plasmid was derived from a plasmid made by: Prof. Markus Schmid (Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, Umeå, SE-901 87, Sweden)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry