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Addgene

pYY1704
(Plasmid #198421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGreen
  • Vector type
    Plant Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RedNTF
  • Species
    Synthetic
  • Promoter pBs3

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGTG
  • 3′ sequencing primer CAGGAAACAGCTATGACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYY1704 was a gift from Yuan You (Addgene plasmid # 198421 ; http://n2t.net/addgene:198421 ; RRID:Addgene_198421)

  • For your References section:

    The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence. You Y, Koczyk G, Nuc M, Morbitzer R, Holmes DR, von Roepenack-Lahaye E, Hou S, Giudicatti A, Gris C, Manavella PA, Noel LD, Krajewski P, Lahaye T. Nat Plants. 2023 Jan;9(1):128-141. doi: 10.1038/s41477-022-01302-y. Epub 2022 Dec 22. 10.1038/s41477-022-01302-y PubMed 36550363
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