pCin4-3xFLAG-MKL1 S449A
(Plasmid
#19842)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19842 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCin4
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Backbone manufacturerS Rees et al., BioTechniques 1996
- Backbone size w/o insert (bp) 5257
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMKL1 S449A
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Alt nameMAL, BSAC, MRTF-A
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SpeciesH. sapiens (human)
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Insert Size (bp)2800
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Mutationchanged Serine 449 to Alanine
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Entrez GeneMRTFA (a.k.a. BSAC, MAL, MKL, MKL1, MRTF-A)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The 3xFlag in the vector is from Sigma, p3xFlag-CMV-7.1 has variant flag sequences (DYKDHD) and is recognized well by their anti-flag monoclonal M2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCin4-3xFLAG-MKL1 S449A was a gift from Ron Prywes (Addgene plasmid # 19842 ; http://n2t.net/addgene:19842 ; RRID:Addgene_19842) -
For your References section:
Serum-induced phosphorylation of the SRF coactivator MKL1 by the ERK1/2 pathway inhibits its nuclear localization. Muehlich S, Wang R, Lee SM, Lewis TC, Dai C, Prywes R. Mol Cell Biol. 2008 Aug 11. ():. 10.1128/MCB.00427-08 PubMed 18694962
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/56/42/7964ed4a-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)