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PB-TO-oNGN2
(Plasmid #198397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198397 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUCM
  • Backbone size w/o insert (bp) 13243
  • Total vector size (bp) 14098
  • Modifications to backbone
    x y z
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    100µg/mL
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    NEUROG2 (phospho mutant, codon optimized)
  • Alt name
    hNGN2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    819
  • Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S242A
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
  • Promoter TRE3G

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHi (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer ccgtaccacttcctaccctc
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    puroR-T2A-mycNLS-mTagBFP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1476
  • Promoter EF1a
  • Tag / Fusion Protein
    • T2A-mycNLS-mTagBFP2

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SwaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    rtTA3G
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    748
  • Promoter CAG

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer ctggttattgtgctgtctc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TO-oNGN2 was a gift from iPSC Neurodegenerative Disease Initiative (iNDI) & Michael Ward (Addgene plasmid # 198397 ; http://n2t.net/addgene:198397 ; RRID:Addgene_198397)
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