Skip to main content
Addgene

pLX304 3xFlag-eRF1 G183/184A_IRES-GFP
(Plasmid #198394)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLX304
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin, GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eRF1
  • Alt name
    ETF1
  • Species
    H. sapiens (human)
  • Mutation
    mutated GGQ motif (G183/184A)
  • Entrez Gene
    ETF1 (a.k.a. D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304 3xFlag-eRF1 G183/184A_IRES-GFP was a gift from Jack Taunton (Addgene plasmid # 198394 ; http://n2t.net/addgene:198394 ; RRID:Addgene_198394)

  • For your References section:

    An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes. Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J. Cell. 2023 Jan 19;186(2):346-362.e17. doi: 10.1016/j.cell.2022.12.025. Epub 2023 Jan 12. 10.1016/j.cell.2022.12.025 PubMed 36638793
Related items:
Commonly requested with: