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Addgene

DNMT2Δ191-237
(Plasmid #198382)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Total vector size (bp) 6328
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use E. coli Rosetta 2 (DE3) pLysS cells for expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DNMT2
  • Alt name
    TRDMT1
  • Alt name
    tRNA aspartic acid methyltransferase 1
  • Species
    H. sapiens (human)
  • Mutation
    deleted amino acids 191-237
  • Entrez Gene
    TRDMT1 (a.k.a. DMNT2, DNMT2, MHSAIIP, PUMET, RNMT1)
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DNMT2Δ191-237 was a gift from Tanja Schirmeister (Addgene plasmid # 198382 ; http://n2t.net/addgene:198382 ; RRID:Addgene_198382)

  • For your References section:

    Covalent S-Adenosylhomocysteine-Based DNA Methyltransferase 2 Inhibitors with a New Type of Aryl Warhead. Schwickert M, Zimmermann RA, Habeck T, Hoba SN, Nidoieva Z, Fischer TR, Stark MM, Kersten C, Lermyte F, Helm M, Schirmeister T. ACS Med Chem Lett. 2023 May 9;14(6):777-787. doi: 10.1021/acsmedchemlett.3c00062. eCollection 2023 Jun 8. 10.1021/acsmedchemlett.3c00062 PubMed 37312859
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