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pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]
(Plasmid #198381)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198381 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2CG
  • Backbone size w/o insert (bp) 5883
  • Total vector size (bp) 10209
  • Vector type
    Tol2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UAS:sypb-egfp-cry2
  • Alt name
    Sypb-EGFP-Cry2(D387A)olig(535)
  • Species
    D. rerio (zebrafish), A. thaliana (mustard weed)
  • Insert Size (bp)
    3998
  • Mutation
    D387A
  • GenBank ID
    ENSDARG00000002230
  • Promoter UAS
  • Tag / Fusion Protein
    • EGFP

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer gaccatgattacgccaagct
  • 3′ sequencing primer gtaaaacgacggccagtgaa
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA] was a gift from Alexander Gottschalk (Addgene plasmid # 198381 ; http://n2t.net/addgene:198381 ; RRID:Addgene_198381)

  • For your References section:

    Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles. Vettkotter D, Schneider M, Goulden BD, Dill H, Liewald J, Zeiler S, Guldan J, Ates YA, Watanabe S, Gottschalk A. Nat Commun. 2022 Dec 19;13(1):7827. doi: 10.1038/s41467-022-35324-z. 10.1038/s41467-022-35324-z PubMed 36535932
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