N-Myc-53BP1 WT pLPC-Puro
(Plasmid #19836)

• Depositing Lab: Titia de Lange
• Publication: Dimitrova et al Nature. 2008 Oct 19. ():.

Backbone

• Vector backbone: pLPC
• Backbone manufacturer: Scott Lowe
• Backbone size w/o insert (bp): 5600
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: 30C Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53 Binding Protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 5900
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TAGGCGTGTACGGTGGGA
• 3′ sequencing primer: CTTGCCAAACCTACAGGT

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest for 19836
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There may be a ~1.5kb band on a gel. This band is likely the result of a recombination product because of
the size of the construct. The maxipreps
probably contain two plasmids -- the full length and a short version that has
still retained the amp resistance gene but lost part of the construct.