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pGADT7-AP-2 σ2
(Plasmid #198345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGADT7
  • Backbone size w/o insert (bp) 7987
  • Total vector size (bp) 8382
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AP-2 σ2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    428
  • Mutation
    R42G substitution, and silent substitutions in codons 3, 4, 5, 138 and 140
  • Promoter ADH1
  • Tags / Fusion Proteins
    • GAL4 transcriptional activation domain (AD) fragment (N terminal on backbone)
    • HA tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GAL4 transcriptional activation domain forward
  • 3′ sequencing primer GAL4 transcriptional activation domain reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGADT7-AP-2 σ2 was a gift from Juan Bonifacino (Addgene plasmid # 198345 ; http://n2t.net/addgene:198345 ; RRID:Addgene_198345)

  • For your References section:

    The adaptor protein chaperone AAGAB stabilizes AP-4 complex subunits. Mattera R, De Pace R, Bonifacino JS. Mol Biol Cell. 2022 Oct 1;33(12):ar109. doi: 10.1091/mbc.E22-05-0177. Epub 2022 Aug 17. 10.1091/mbc.E22-05-0177 PubMed 35976721
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