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Addgene

Dyn1 R271E
(Plasmid #198343)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198343 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dynamin1
  • Species
    H. sapiens (human)
  • Mutation
    R271E
  • Entrez Gene
    DNM1 (a.k.a. DEE31, DNM, EIEE31)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dyn1 R271E was a gift from Justin Taraska (Addgene plasmid # 198343 ; http://n2t.net/addgene:198343 ; RRID:Addgene_198343)

  • For your References section:

    Cryo-EM structures of membrane-bound dynamin in a post-hydrolysis state primed for membrane fission. Jimah JR, Kundu N, Stanton AE, Sochacki KA, Canagarajah B, Chan L, Strub MP, Wang H, Taraska JW, Hinshaw JE. Dev Cell. 2024 Apr 23:S1534-5807(24)00233-8. doi: 10.1016/j.devcel.2024.04.008. 10.1016/j.devcel.2024.04.008 PubMed 38663399
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