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Addgene

PB-Ef1a-RPS27A-UTR-PP7-WPRE
(Plasmid #198339)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    L50
  • Backbone manufacturer
    in house
  • Backbone size w/o insert (bp) 5792
  • Total vector size (bp) 8115
  • Vector type
    Mammalian Expression ; PiggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RPS27A-3'UTR-PP7 stem loops x24
  • Alt name
    RPS27A-PP7
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2232
  • Promoter Ef1a
  • Tag / Fusion Protein
    • PP7 stem loops (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer AATTGTCAGTGCCCAACAGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-Ef1a-RPS27A-UTR-PP7-WPRE was a gift from Michael Ward (Addgene plasmid # 198339 ; http://n2t.net/addgene:198339 ; RRID:Addgene_198339)

  • For your References section:

    Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration. De Pace R, Ghosh S, Ryan VH, Sohn M, Jarnik M, Rezvan Sangsari P, Morgan NY, Dale RK, Ward ME, Bonifacino JS. Nat Neurosci. 2024 Apr 10. doi: 10.1038/s41593-024-01619-1. 10.1038/s41593-024-01619-1 PubMed 38600167
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