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Addgene

p-mCherry2-sgC9cen
(Plasmid #198326)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198326 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmCherry-sgRNA (empty)
  • Backbone manufacturer
    Addgene 198330
  • Backbone size w/o insert (bp) 5258
  • Total vector size (bp) 5133
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chr9-CEN guide RNA
  • gRNA/shRNA sequence
    TGGAATGGAATGGAATGGAA
  • Insert Size (bp)
    25
  • Promoter hU6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer tacaccatcgtggaacagta
  • 3′ sequencing primer gaggtgtgggaggtttttta
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Chr9-CEN target sequence: TGGAATGGAATGGAATGGAA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-mCherry2-sgC9cen was a gift from Sarah McClelland (Addgene plasmid # 198326 ; http://n2t.net/addgene:198326 ; RRID:Addgene_198326)
  • For your References section:

    Targeted assembly of ectopic kinetochores to induce chromosome-specific segmental aneuploidies. Tovini L, Johnson SC, Guscott MA, Andersen AM, Spierings DCJ, Wardenaar R, Foijer F, McClelland SE. EMBO J. 2023 May 15;42(10):e111587. doi: 10.15252/embj.2022111587. Epub 2023 Apr 17. 10.15252/embj.2022111587 PubMed 37063065