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pKE13-ISceI-fabp10a:CreERT2
(Plasmid #198242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Zebrafish transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    6344
  • Entrez Gene
    fabp10a (a.k.a. L-FABP, Lb-FABP, Lfabp, Zf-FABP10, fabp10, z-L-BABP, zgc:103719, zgc:92741)

Gene/Insert 2

  • Gene/Insert name
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1516
  • Entrez Gene
    cryaa (a.k.a. wu:fc06h04, zgc:92036)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The CreERT2 sequence is derived from TP1-CreERT2 (Nikolay Ninov) The remainder of the sequence is derived from the pHD157 plasmid (Didier Stainier and Daniel Hesselson).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note there is mcherry sequence downstream of fabp10a promoter, but it does not produce a functional (fluorescent) protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKE13-ISceI-fabp10a:CreERT2 was a gift from Didier Stainier (Addgene plasmid # 198242 ; http://n2t.net/addgene:198242 ; RRID:Addgene_198242)

  • For your References section:

    Heterogeneous beta-catenin activation is sufficient to cause hepatocellular carcinoma in zebrafish. Kalasekar SM, Kotiyal S, Conley C, Phan C, Young A, Evason KJ. Biol Open. 2019 Oct 17;8(10):bio047829. doi: 10.1242/bio.047829. 10.1242/bio.047829 PubMed 31575545
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