pKE13-ISceI-fabp10a:CreERT2
(Plasmid #198242)

• Purpose: For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase
• Depositing Lab: Didier Stainier
• Publication: Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829. doi: 10.1242/bio.047829.

Backbone

• Vector backbone: Unknown
• Vector type: Zebrafish transgenesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA
• Species: D. rerio (zebrafish)
• Insert Size (bp): 6344
• Entrez Gene: fabp10a (a.k.a. L-FABP, Lb-FABP, Lfabp, Zf-FABP10, fabp10, z-L-BABP, zgc:103719, zgc:92741)

Gene/Insert 2

• Gene/Insert name: Zebrafish cryaa promoter:Venus fluorescent protein coding sequence
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1516
• Entrez Gene: cryaa (a.k.a. wu:fc06h04, zgc:92036)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The CreERT2 sequence is derived from TP1-CreERT2 (Nikolay Ninov) The remainder of the sequence is derived from the pHD157 plasmid (Didier Stainier and Daniel Hesselson).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note there is mcherry sequence downstream of fabp10a promoter, but it does not produce a functional (fluorescent) protein.