pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
(Plasmid #198240)

• Purpose: For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites
• Depositing Lab: Didier Stainier
• Publication: Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829. doi: 10.1242/bio.047829.

Backbone

• Vector backbone: Unknown
• Vector type: Zebrafish transgenesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA
• Species: X. laevis (frog)
• Insert Size (bp): 5280
• Entrez Gene: fabp10a (a.k.a. L-FABP, Lb-FABP, Lfabp, Zf-FABP10, fabp10, z-L-BABP, zgc:103719, zgc:92741)

Gene/Insert 2

• Gene/Insert name: Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1516
• Entrez Gene: cryaa (a.k.a. wu:fc06h04, zgc:92036)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Xenopus pt-beta-catenin sequence is derived from pCS2-XE49 (Randall Moon lab). The remainder of the sequence is derived from the pHD157 and pHD142 plasmids (Didier Stainier and Daniel Hesselson).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry