pCW57.1-3xFLAG-NLS-GFP
(Plasmid
#198238)
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PurposeLentivector with tet-inducible 3xFLAG-NLS-GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198238 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCW57.1
- Backbone size w/o insert (bp) 9354
- Total vector size (bp) 8744
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsWe used Stbl3 cells (recombination deficient)
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xFLAG-NLS-GFP
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Insert Size (bp)828
- Promoter TRE
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer aggtaggcgtgtacggtgg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCW57.1-3xFLAG-NLS-GFP was a gift from Stephen Tapscott (Addgene plasmid # 198238 ; http://n2t.net/addgene:198238 ; RRID:Addgene_198238) -
For your References section:
Human DUX4 and porcine DUXC activate similar early embryonic programs in pig muscle cells: implications for preclinical models of FSHD. Nip Y, Bennett SR, Smith AA, Jones TI, Jones PL, Tapscott SJ. Hum Mol Genet. 2023 Feb 2:ddad021. doi: 10.1093/hmg/ddad021. 10.1093/hmg/ddad021 PubMed 36728804