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pCW57.1-pDUXChd:hDUX4ctd-ca
(Plasmid #198237)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198237 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1
  • Backbone size w/o insert (bp) 9354
  • Total vector size (bp) 9100
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    We used Stbl3 cells (recombination deficient)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DUX4L pDUXChd:hDUX4ctd (codon altered)
  • Alt name
    porcine DUXC/human DUX4 chimera (codon altered)
  • Species
    H. sapiens (human); S. scrofa (porcine)
  • Insert Size (bp)
    1320
  • Mutation
    codon altered
  • Entrez Gene
    DUX4 (a.k.a. DUX4L)
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer aggtaggcgtgtacggtgg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1-pDUXChd:hDUX4ctd-ca was a gift from Stephen Tapscott (Addgene plasmid # 198237 ; http://n2t.net/addgene:198237 ; RRID:Addgene_198237)

  • For your References section:

    Human DUX4 and porcine DUXC activate similar early embryonic programs in pig muscle cells: implications for preclinical models of FSHD. Nip Y, Bennett SR, Smith AA, Jones TI, Jones PL, Tapscott SJ. Hum Mol Genet. 2023 Feb 2:ddad021. doi: 10.1093/hmg/ddad021. 10.1093/hmg/ddad021 PubMed 36728804
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