pBUE411-GG
(Plasmid #198232)

• Purpose: Wheat GRF4-GIF1 chimera in pBUE411
• Depositing Lab: Andrea Gallavotti
• Publication: The combination of morphogenic regulators BABY BOOM and GRF-GIF improves maize transformation efficiency (unpublished)

Backbone

• Vector backbone: pBUE411
• Backbone size w/o insert (bp): 6500
• Total vector size (bp): 21757
• Vector type: Plant Expression, CRISPR ; plant binary vector
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GRF4-GIF1 chimera cassette
• Species: Synthetic; Wheat; Agrobacterium tumefaciens
• Insert Size (bp): 4367
• Promoter: Ubiquitin

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcgtttcccgccttcagtttGAGCTCGGTACCCGGGGA
• 3′ sequencing primer: ctgtcaaacactgatagtttCTGCAGGCGCGCTAATTCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The GRF4-GIF1 chimera was obtained from the laboratory of Jorge Dubcovsky at the University of California Davis and Howard Hughes Medical Institute (Addgene deposit JD553), and is described in the following publication: Debernardi et al Nat Biotechnol. 2020 Oct 12. doi: 10.1038/s41587-020-0703-0.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.09.02.506370v1 for bioRxiv preprint.