pMX-Mef2c-puro
(Plasmid
#198228)
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PurposeRetroviral expression of Mef2c
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMX
- Backbone size w/o insert (bp) 5600
- Total vector size (bp) 6900
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMef2c
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SpeciesM. musculus (mouse)
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Insert Size (bp)1257
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Entrez GeneMef2c (a.k.a. 5430401D19Rik, 9930028G15Rik, Mef2)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe plasmid is originally published in Ieda, Masaki, et al. "Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors." Cell 142.3 (2010): 375-386.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX-Mef2c-puro was a gift from Li Qian (Addgene plasmid # 198228 ; http://n2t.net/addgene:198228 ; RRID:Addgene_198228) -
For your References section:
Cross-lineage potential of Ascl1 uncovered by comparing diverse reprogramming regulatomes. Wang H, Keepers B, Qian Y, Xie Y, Colon M, Liu J, Qian L. Cell Stem Cell. 2022 Oct 6;29(10):1491-1504.e9. doi: 10.1016/j.stem.2022.09.006. 10.1016/j.stem.2022.09.006 PubMed 36206732