pHR_SFFV*/Puro2a-PAG1-GFP
(Plasmid #198223)

• Purpose: Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
• Depositing Lab: Eric Davis
• Publication: Transposon vectors for proximity biotin labeling with split TurboID (unpublished)

Backbone

• Vector backbone: pHR_SFFV
• Backbone manufacturer: Addgene #79121
• Backbone size w/o insert (bp): 8500
• Total vector size (bp): 11300
• Modifications to backbone: The unique NcoI and SfiI sites within the SV40 promoter were opened, releasing a short sequence, and filled in with an oligo that restored the NcoI site, and also restored the removed sequence, with the exception of adding 2 nucleotides that destroyed the SfiI site and created a KpnI site.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PAG1
• Alt name: CBP
• Species: H. sapiens (human)
• Insert Size (bp): 2800
• Mutation: None
• GenBank ID: NM_018440.4
• Entrez Gene: PAG1 (a.k.a. CBP, PAG)
• Tags / Fusion Proteins:
  • Preceded by puromycin resistance gene, before an FMVD 2A peptide (N terminal on insert)
  • Followed by EGFP, after a flexible linker (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The PAG1 cDNA, and the human codon-optimized puromycin resistance gene with FMVD 2A, were separately synthesized as gene blocks by IDT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry