pSBtet-Bla/PAG1-GFP
(Plasmid #198220)

• Purpose: Transposon vector (Addgene #60510) for doxycycline-inducible expression of human PAG1 fused to EGFP
• Depositing Lab: Eric Davis
• Publication: Transposon vectors for proximity biotin labeling with split TurboID (unpublished)

Backbone

• Vector backbone: pSBtet-Bla
• Backbone manufacturer: Addgene #60510
• Backbone size w/o insert (bp): 7037
• Total vector size (bp): 9100
• Modifications to backbone: None
• Vector type: Mammalian Expression ; Transposon
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PAG1
• Alt name: CBP
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: None
• GenBank ID: NM_018440.4
• Entrez Gene: PAG1 (a.k.a. CBP, PAG)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene block synthesized by IDT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry