pSBbi-Pur/GDI1-splitTurboID
(Plasmid
#198219)
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PurposeTransposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labeling
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSBbi-Pur
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Backbone manufacturerAddgene #60523
- Backbone size w/o insert (bp) 5857
- Total vector size (bp) 7500
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Modifications to backboneNone
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Vector typeMammalian Expression ; Transposon
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGID1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1032
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Tag
/ Fusion Protein
- 5’ part of TurboID, up to E256 (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHuman codon-optimized gene block synthesized by IDT
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBbi-Pur/GDI1-splitTurboID was a gift from Eric Davis (Addgene plasmid # 198219 ; http://n2t.net/addgene:198219 ; RRID:Addgene_198219)