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Addgene

pSBtet-Bla/mCardinal-TurboID-GAI
(Plasmid #198218)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198218 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSBtet-Bla
  • Backbone manufacturer
    Addgene #60510
  • Backbone size w/o insert (bp) 7037
  • Total vector size (bp) 8400
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCardinal
  • Species
    Synthetic
  • Insert Size (bp)
    735
  • Mutation
    None
  • GenBank ID
  • Tags / Fusion Proteins
    • 3’ part of TurboID, starting with G257 (C terminal on insert)
    • amino acids 11-93 of GAI (gibberellin insensitive)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene blocks synthesized by IDT

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBtet-Bla/mCardinal-TurboID-GAI was a gift from Eric Davis (Addgene plasmid # 198218 ; http://n2t.net/addgene:198218 ; RRID:Addgene_198218)