pSBtet-Bla/mCardinal-TurboID-GAI
(Plasmid
#198218)
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PurposeTransposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSBtet-Bla
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Backbone manufacturerAddgene #60510
- Backbone size w/o insert (bp) 7037
- Total vector size (bp) 8400
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Modifications to backboneNone
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Vector typeMammalian Expression ; Transposon
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCardinal
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SpeciesSynthetic
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Insert Size (bp)735
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MutationNone
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GenBank ID
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Tags
/ Fusion Proteins
- 3’ part of TurboID, starting with G257 (C terminal on insert)
- amino acids 11-93 of GAI (gibberellin insensitive)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGene blocks synthesized by IDT
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBtet-Bla/mCardinal-TurboID-GAI was a gift from Eric Davis (Addgene plasmid # 198218 ; http://n2t.net/addgene:198218 ; RRID:Addgene_198218)