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Addgene

pACT2-μ3B
(Plasmid #198175)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACT2
  • Backbone size w/o insert (bp) 8117
  • Total vector size (bp) 9370
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AP-3 μ3B
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1257
  • Promoter ADH1
  • Tags / Fusion Proteins
    • GAL4 transcriptional activation domain (AD) fragment (N terminal on backbone)
    • HA tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer GAL4 transcriptional activation domain (AD) forward
  • 3′ sequencing primer GAL4 transcriptional activation domain (AD) reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACT2-μ3B was a gift from Juan Bonifacino (Addgene plasmid # 198175 ; http://n2t.net/addgene:198175 ; RRID:Addgene_198175)

  • For your References section:

    The adaptor protein-1 mu1B subunit expands the repertoire of basolateral sorting signal recognition in epithelial cells. Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino JS. Dev Cell. 2013 Nov 11;27(3):353-66. doi: 10.1016/j.devcel.2013.10.006. 10.1016/j.devcel.2013.10.006 PubMed 24229647
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