p6187
(Plasmid
#198158)
-
Purposebacterial expression of sifA::HaloTag fusion, mutant form
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 198158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepWSK29
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namePsifA sifA delta331-336::L16::HaloTag::HA
-
SpeciesSalmonella enterica serovar Typhimurium
-
Entrez GenesifA (a.k.a. STM1224)
-
Tag
/ Fusion Protein
- HaloTag-HA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p6187 was a gift from Michael Hensel (Addgene plasmid # 198158 ; http://n2t.net/addgene:198158 ; RRID:Addgene_198158)